|
KMID : 0545120090190090982
|
|
Journal of Microbiology and Biotechnology
2009 Volume.19 No. 9 p.982 ~ p.986
|
|
|
Evaluation of Optimal Culture Conditions for Recombinant Ghost Bacteria Vaccine Production with the Antigen of Streptococcus iniae GAPDH
|
|
Ra Chae-Hun
Kim Yeong-Jin
Park So-Jin
Jeong Chang-Wha
Nam Yoon-Kwon
Kim Ki-Hong
Kim Sung-Koo
|
|
|
Abstract
|
|
|
|
For the production of ghost bacteria vaccine to prevent the streptococcal disease in aquaculture fish species, a double cassettes vector was constructed and cloned in Escherichia coli DH5￥a. Ghost bacteria vaccine production from Escherichia coli DH5￥a/pHCE-InaN-GAPDH-Ghost 37 SDM (SIG) was maximized at a glucose concentration of 1 g/l, agitation of 300 rpm, and aeration of 1 vvm. The maximal efficiency of ghost bacteria formation was obtained at the mid-exponential phase (OD600=2.0) with the concentration of 0.77 g/l for SIG. The molecular mass of GAPDH was detected at 67 kDa with the insoluble fraction, by SDS-PAGE and Western blot. The protective efficacy of ghost bacteria vaccine was evaluated by challenge test using olive flounder. The cumulative mortalities of the positive control, formalin-killed cell (FKC) vaccine, and SIG vaccine immunized groups were 91%, 74%, and 57%, respectively. These results suggest that SIG vaccine showed efficacy as a vaccine and had a higher potential to induce protective antibodies than did FKC vaccine.
|
|
|
KEYWORD
|
|
|
Ghost bacteria vaccine, S. iniae, GAPDH, challenge test
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|